Class Notes (834,979)
Canada (508,839)
Biology (1,909)
BIOL 3010 (47)
Lecture

Lecture 04 Embryonic

2 Pages
37 Views
Unlock Document

Department
Biology
Course
BIOL 3010
Professor
Scott Schau
Semester
Fall

Description
Outline of Lecture 04 Embryonic Manipulations I. Analysis of Gene Expression A) RNA expression: in situ hybridization - Fix sample, incubate with RNA probe, detect probe B) Protein expression: immunohistochemistry - Fix sample, incubate with primary Ab, wash, incubate with and detect secondary Ab C) Gene expression: reporter genes - Often use LacZ gene (β-galactosidase) or green fluorescent protein (GFP) - Fuse with gene of interest or create construct with sequences controlling expression of gene of interest II. Transgenesis: Pronuclear Injection of DNA A) Procedure: inject DNA directly into nucleus, implant in pseudopregnant mouse, detect transgenic mice (e.g. southern blot) and breed B) Random insertion and requires many embryos, but is fast C) Applications - Deletion constructs of regulatory sequences to determine necessary sequences - Use alternative sequences controlling expression to get gene expressed in location where it is normally not - Test genes that whose function is eliminated by dominant-negative effect - Use reporter gene to mark a cell and allow identification at different stages - Mak
More Less

Related notes for BIOL 3010

Log In


OR

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


OR

By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.


Submit