EQN 3060 Lecture Notes - Lecture 13: Pregnancy Rate, Pipette, Glycerol

• School: University of Guelph
• Department: Equine
• Course: EQN 3060
• Professor: Dr. Merkies

Document Summary

Two types of preservation: 1) liquid storage 2) cryopreservation: liquid preservation. Collect and evaluate semen: viability, motility, morphology (70% reduces to approximately 60% after freezing, any less needs to be considered for other ways of insemination, such as natural) Extend semen provides the following: energy (cid:374)eed to pro(cid:448)ide (cid:862)food(cid:863) (cid:894)glu(cid:272)ose(cid:895, electrolytes sodiu(cid:373), potassiu(cid:373), (cid:272)al(cid:272)iu(cid:373)/salts; help regulate (cid:449)hat (cid:858)goes(cid:859) into and out of the cell to maintain balance. Package: example: equitainer (can keep semen viable for up to 72 hours) cools semen at a specific rate to prevent cold-shock. Insemination dose: 500 million progressively motile sperm (ai: the equitainer (, two frozen cans are placed in the bottom of the container (frozen liquid on top of the can) to monitor the rate of cooling. Isothermal cup with semen sample is placed in the equitainer: semen sample is carefully cooled at the proper rate to cool it 5 degrees.