MCB 2050 Lecture Notes - Lecture 2: Avidin, Expressed Sequence Tag, Restriction Enzyme

• School: University of Guelph
• Department: Molecular and Cellular Biology
• Course: MCB 2050
• Professor: ALL

Document Summary

Overview of cytological, physical and genetic maps: cytological map, genetic map, physical map, contig map, genomic sequence. Structural genomics: cytological mapping: shows g bands in human karyotype, mitotic chromosome smear, treat with the protease trypsin, stain with giemsa, observe by light microscopy, photograph and arrange chromosomes. Fish: fluorescence in situe hybridization: hybridize biotin-bound dna probe to cells on a glass slide, microscopic observation with fluorescence microscope. Incubate with avidin bound to a fluorescent dye: telomeres detected by fish. Chromosome painting by fish: different chromosome specific probes can be labelled with different fluorescent dyes (e. g. yellow, red, green, blue etc) Structural genomics: physical mapping: physical maps, map based on physical non-polymorphic (sequence specific) markers: actual molecular distances measured in base pairs, higher resolution (in 100kb or smaller) Identifying/ordering contigs by sts/est mapping: to map an est or sts to specific cloned dnas, generate the contig map based on whether 2 or more clones share the same est or sts.