MCB 2050 Lecture Notes - Lecture 8: Organelle, Molecular Motor, Integral Membrane Protein
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23 Aug 2018
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Endomembrane system: protein trafficking: fate of a newly-synthesized protein in the er, nascent, properly folded and glycosylated soluble or membrane protein in the rer is either , retained in the er, Or: exits the er, [moves to the er exit sites (eres) = er subdomain involved in the formation of transport vesicles destined for the golgi] Cop serve two main functions: (easy question: recognize and concentrate specific protein (& lipid) components that will be incorporated into the budding vesicle, mediate eres membrane curvature & the formation of the budding vesicle. The three different coat proteins: copii, copi, clathrin. Step 1: soluble copii component sar1-gdp (sar1 = g protein) is recruited from the cytosolic surface (outer leaflet) of the. Eres: sar1-gdp binds a guanine-exchange factor (gef), which generates sar1-gtp starts to make membrane curve, gef = an er integral membrane protein that catalyzes the exchange of gdp for gtp on sar1.