MCB 2050 Lecture Notes - Lecture 1: Hybridization Probe, Nylon, Digestion

• School: University of Guelph
• Department: Molecular and Cellular Biology
• Course: MCB 2050
• Professor: Penny

Document Summary

Const. of dna libraries major steps: digestion of vector cut vector w restriction enz w/in the multiple clonins site, digest geonomic dna , annealing and ligation, transformation. Clone library : cdna libs copies of all mrnas in a cell) complimentary sequence to your rna. Molecular hybridization: radio act dna or rna labelles as a hybrid probe to id comp dna bout to a nylon membrane. Smmmary slide: screenikn dna libs by colony hybridization usung radioactive cdna as a hybridization probe.