MICR 2420 Lecture Notes - Lecture 11: Lipid Bilayer Fusion, Retrovirus, Optical Microscope

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Entry by mechanical transmission: enter by transmission through 1)an insect vector (transmits disease, 2)infected seed (e. g gemini virus introduced by beetles, grasshoppers, 3)damaged tissues- entry into damaged cells non specific (tobacco mosaic virus) Plants have thick complex cell walls that prevent lytic burst or budding of virions. Hence transmission is via plasmodesmata (membrane & er channels) Culturing viruses: need a host cell (eukaryote or prokaryote) There are 2 kinds of bacteriophage culture systems: Isolate plaques on bacterial lawn (agar plate) high yield with high volumes. Plaques are formed when host cells get destroyed in culture= cyticidal effects of viral replication. 4- virus reproduces, host cells lyse and plaques formed. Cytopathic effect = structural change of host cell due to viral infection by citocidal virus e. g: poliovirus replication in human tissue culture cells. Fluorescent dyes may bind cellular structures (e. g dapi stain with dna) or. May be covalently linked to molecular probes that bind cellular structures.

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