MICR 3220 Lecture Notes - Lecture 22: Pilus, Agrobacterium, Endonuclease
30 Dec 2015
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Two component regulatory system vira and virg, roles in membrane and cytoplasm, attaching to promoter element to turn on remaining genes, vir genes located on ti (tumor inducing) plasmid. Bacteria knows its at wound site to start infection. We need to get tdna? out of bacteria and into chromosome of host plant. Bacteria will genetically engineer plant tdna has borders on it, the left and right border inbetween is the tdna outside is the regular parts of plasmid. Vir d protein is a type of endonuclease which means it can digest dna, migrates and binds to right border, right and left border has specific sequences called direct repeats. Also makes nick in left border the difference between right and left. Multiple vird proteins, will bind to repeat on right border and has. Dna the vird protein actually remains attached to bottom strand of the. When it peels it back it separates the bottom strand away from.
