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Class Notes for Microbiology at University of Guelph

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MICR 4280 Lecture Notes - Lecture 8: Sybr Green I, Taqman, Real-Time Polymerase Chain Reaction
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FRIDAY JANUARY 25 TH LECTURE 8: QPCR THERMOCYCLER: For TaqMan and Syber-green qPCR: expose reaction mixture to wavelength of excitation of fluorescent reporterdye (R or Syber Green) and monitor fluorescence emissions in re...

Microbiology
MICR 4280
Wendy Keenleyside
MICR 4280 Lecture Notes - Lecture 6: Shotgun Sequencing, 16S Ribosomal Rna, Axenic
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MONDAY JANUARY 21RST LECTURE 6 AXENIC CULTURES: Axenic cultures are critical for basic and applied microbiology o Accommodating unknown environmental requirements: using in situ cultivation o Common principle: diffusion th...

Microbiology
MICR 4280
Wendy Keenleyside
MICR 4280 Lecture Notes - Lecture 7: Axenic, Microbial Ecology, 16S Ribosomal Rna
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WEDNESDAY JANUARY 23 RD LECTURE 7 GENOMIC APPROACHES Bottom up means you have an axenic culture of the organism, vs top-down. o Axenic culture = pure culture Sequence the genome, purify all the messenger RNA, use viral enz...

Microbiology
MICR 4280
Wendy Keenleyside
MICR 4280 Lecture 5: Lecture 5
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FRIDAY JANUARY 18 TH LECTURE 5: GAIA HYPOTHESIS This operates on geological time scales, we may be long dead before things regulate again The default is ice-age, we are just lucky enough to be in a Gaia theory state accord...

Microbiology
MICR 4280
Wendy Keenleyside
MICR 3220 Lecture Notes - Lecture 26: Root Rot, Zoospore, Armillaria
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WEDNESDAY NOVEMBER 14 : TH The hyphae growing into the root tissue is what causes the rotting This causes the production of sporangia (the asexual spores for oomycetes and oopsores the sexual spore). These can be formed in...

Microbiology
MICR 3220
Paul Goodwin
MICR 3220 Lecture Notes - Lecture 31: Barley Yellow Dwarf, Open Reading Frame, Orf Eins
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MONDAY NOVEMBER 26 : TH The genome is 5000-1000kb the smallest excluding viruses o Estimated that 75 of the genes lost o Seen in bacillus and clostridium This makes it very difficult to culture, this makes it limited to a ...

Microbiology
MICR 3220
Paul Goodwin
MICR 3220 Lecture 25: Lecture 25
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MONDAY NOVEMBER 12 TH Mechanisms of biological control o Reduce pathogen inoculum Resource competition: if you can get saprophytes to obtain nutrients you can starve out the pathogens, as saprophytes will absorb the nutrie...

Microbiology
MICR 3220
Paul Goodwin
MICR 3220 Lecture 32: Lecture 32
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MONDAY NOVEMBER 28 TH Endophytes: non-pathogenic organisms that live inside of plants. We are interested in them because of there mutualistic behaviors Choke disease: most of the time it is not bad for the plant until flow...

Microbiology
MICR 3220
Paul Goodwin
MICR 3220 Lecture Notes - Lecture 29: Xylem, Fusarium, Pectin
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WEDNESDAY OCTOBER 21RST: The microconidia float up in the sap stream, but they run into the xylem end walls which is a problem o The pores in the xylem end walls are too small for the microconidia to pass o These microconi...

Microbiology
MICR 3220
Paul Goodwin
MICR 3220 Lecture Notes - Lecture 28: Hartig Net, Basidiomycota, Drought Tolerance
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MONDAY NOVEMBER 19 TH Benefits: o Drought tolerance: hyphae are able to take up water, the water moves up from the hyphae to the Arbuscule and through the plant extends the absorptive zone If you inoculate plants with thes...

Microbiology
MICR 3220
Paul Goodwin
MICR 3220 Lecture 27: Lecture 27
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FRIDAY NOVEMBER 16 : TH Life cycle: LCOs cause irregular growth of the root hair, the root hair curl and the bacteria get caught in this curl and will start to invaginate. The LCOs reorganize the cytoplasm of the root cell...

Microbiology
MICR 3220
Paul Goodwin
MICR 3220 Lecture 30: Lecture 30
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FRIDAY NOVEMBER 23 : RD Bacteria: called the phytoplasmas (wall-less bacteria that are specialized just for the seive tubes) o Last group of pathogens to be identified, this took a long time because the symptoms caused by ...

Microbiology
MICR 3220
Paul Goodwin
MICR 3220 Lecture 20: Lecture 20
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find more resources at oneclass.com MONDAY OCTOBER 29 TH Open reading frame one: no need for an expression strategy because it is located at 5 end so it can be read normally o ...

Microbiology
MICR 3220
Paul Goodwin
MICR 3220 Lecture 19: Lecture 19
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find more resources at oneclass.com FRIDAY OCTOBER 26 TH Plant viruses: are obligate biotrophs because they do not have an independent metabolism o Composed of a nucleic acid and a prot...

Microbiology
MICR 3220
Paul Goodwin
MICR 3220 Lecture Notes - Lecture 18: Conidium, Basidiomycota, Ascocarp
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MONDAY OCTOBER 22 ND : Barley: o We grow winter barley, by the end of the spring the barley is harvested enables you to harvest the crop much sooner because you started in the fall o In the summer time: when over 30 degree...

Microbiology
MICR 3220
Paul Goodwin

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