MBG 3080 Lecture Notes - Lecture 1: Invertase, Integrase, Inverted Repeat
21 Dec 2016
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Document Summary
Can move between parts of the genome or between plasmid and genome. Their insertion affects the functionality of the genes they insert between. Insertion elements: (is: movement catalyzed by transposase (it is i=encoded within the insertion element) In e. coli they don"t transpose all that often: the bigger the sequence the more rare its occurrence in the genome, once excised their previous location can be seen by direct repeats in the genome. Composite transposons (tn10: contains additional genes to those needed for its movement, flanked by 2 is, otherwise moves like is (cut and paste) but tn10 goes into specific target sequence while. Replicative transposition: needs transposase and resolvase to move and copy. Preformed by site-specyfic recombinase (integrase) that recognize specyfic sequences in 2 dna sites and promotes recombination into tehm. Integrons are oftern responsible of racquisition of geomic islands y the bacteriqa. Sample inversion mechanism in sarmonella flagela protein alternation.
