MCB 2050 Lecture Notes - Lecture 2: Electrophoresis, Plasmid, Hindiii

• School: University of Guelph
• Department: Molecular and Cellular Biology
• Course: MCB 2050
• Professor: Dick Mosser

Document Summary

**techniques of molecular genetics (snustad 6e: ch 14)** Electrophoresis uses an electric field to separate macromolecules in agarose (dna/rna) or polyacrylamide (dna or protein) gels. Separation of a mixture of dna (or rna) fragments in porous gel matrix (agarose or polyacrylamide) in an electric field. Since dna is negatively charged, it will migrate toward the anode (positive electrode) Their rates of migration are inversely proportional to their size (i. e. lengths the shorter ones move faster than the longer ones) Applications: determines sizes of dna fragments. *do(cid:374)"t (cid:374)eed to k(cid:374)ow e(cid:454)a(cid:272)t steps just k(cid:374)ow that you make a gel (agarose or dna) wait for it to settle, put in. Dna and it separates based on size as it runs through towards +ve end. Techniques for dna and rna analysis restriction mapping (for physical mapping of dna based on relative location of re sites) Mapping of restriction sites on dna: a(cid:374) e(cid:454)a(cid:373)ple of (cid:862)ph(cid:455)si(cid:272)al (cid:373)appi(cid:374)g(cid:863)