PATH 3610 Lecture Notes - Lecture 3: Lysis, Histopathology, In Situ Hybridization
21 Feb 2014
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Standardized test and reagents of good quality. Miniaturized assays to conserve reagents and reduce costs. Computerized analysis and printouts: objectivity, reporting, record keeping, billing. Put another way, if the test is highly sensitive and the test result is negative you can be nearly certain that they don"t have disease. In other terms, if the test result for a highly specific test is positive you can be nearly certain that they actually have the disease. Detecting active infection: viral culture, histopathology, electron microscopy, detection of viral antigens, detection of viral nucleic acid. Assessing virus-specific immune response: serologic testing. The methods can be broadly divided into 3 categories: those that measure virus infectivity, those that examine viral serology, those that rely on molecular methods. In cultured cells: recognition of virus growth in cell culture: cytophatic effects (rounding, clumping, fusion, syncytium, inclusion body) Each plaque represents 1 pfu (plaque forming unit) Promoters usually attached to detectable gene ( -galactosidase)
