PBIO 4000 Lecture Notes - Lecture 9: Tobacco Mosaic Virus, Endoplasmic Reticulum, Messenger Rna
Document Summary
Small wounds (where plant cell can survive) or insect feeding: need to get into cytoplasm of a functioning plant cell. Plasmadesmata = small cytoplasmic bridges between cells: allows moving from cell to cell. Get into phloem and can spread rapidly throughout the plant, becoming systemic. Genome made out of: rna: ssrna (single stranded) or dsrna (double stranded) 95% are rna genomes: dna: ssdna or dsdna. Positive (+) sense ssrna: can act as a messenger rna (vast majority, trying to get translated into proteins to reproduce in cells, act as rogue mrna . Negative (-) sense ssrna: complementary strand, can knockout directly, need complementary version made and then the complementary version is what is acting in the plant. Approximately 80% of the viruses have the positive sense ssrna genomes. Can have physical separation of genomes, as monopartite or divided (bipartite, tripartite, etc. : monopartite = one strand, or broken up & packaged separately (bipartite = 2 strands, tripartite = 3 strands, etc. )