MBIO 2020 Lecture Notes - Lecture 8: Bacillus Subtilis, Proteome, Transcriptome

• School: University of Manitoba
• Department: Microbiology
• Course: MBIO 2020
• Professor: Anne

Document Summary

Lecture 8- mbio 2020- genome and gene expression. Genome and gene expression: three levels, 1. Genome all dna in a given cell: genomics: study of whole genome, 2. Transcriptome all rnas at a given moment: nucleic acid arrays: study transcription activity at a given time, 3. Proteins involved with gene expression: i. sigma factors, e. coli has six factors: s for proteins needed under starvations conditions, involved in nitrogen metabolism, h and. E for heat shock, f for chemotaxis and d ( 70) for most constitutive and exponential phase genes: b. subtilis has cascade of factors that coordinate differentiation from vegetative growth to sporulation, ii. Regulatory genes= genes whose products regulate the expression of other. Gene expression in e. coli: e. coli chromosome codes for about 4000 genes, only about 800 mrnas are present during growth, gene expression is regulated.