MBIO 2420 Lecture Notes - Lecture 28: Sv40 Large T Antigen, Precursor Mrna, Alternative Splicing

• School: University of Manitoba
• Department: Microbiology
• Course: MBIO 2420
• Professor: Dr.Peter Pelka

Document Summary

All t antigens have the j domain. Large t antigen splices out zn domain: zn domain binds with pp2a, can add or remove phosphates, the small t antigen has this domain. Middle t antigen of mouse polyoma: has a splice variant that shifts the reading frame, has a region that can start a cascade, mapk - starts growth etc . Single late precursor mrna: regulated by large t antigen. Vp1 made from one its own mature mrna. Vp2/vp3 produced from the same mrna by translational control: there is slippage of the ribosome - causes a different reading frame, does not initiation translation at the first start codon, start codon - atg and uag - After viral genome has replicated starts late phase. Only 1 ori site: large t antigen. A cell that has entered a cell cycle, large supply of dntps, replication machinery.