MBIO 2420 Lecture Notes - Lecture 27: Sv40 Large T Antigen, Polyomaviridae, Endoplasmic Reticulum
7 Dec 2016
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Document Summary
Virion contains only protein and nucleic acid: non enveloped. Covalently closed, circular genome of ds dna. Encode three capsid proteins: vp1, vp2, vp3. Encode either two or three tumour antigens: t antigens, from 1 gene - spliced variants. Present in body and always checked by immune system: only become a problem in immune compromised people. From rhesus monkeys: found sv40 - was not killed by treatment, vaccine was already giving to tons of people. Did long term studies for people with vaccine with sv40 and there was no increase of cancer: yay. A good immune system keeps sv40 in check. Vp1 is the one on the outside. Vp2 and vp3 are on the inside. Vp2 attaches with the dna that has histones. Internalized by clathrin, caveolae, or cholesterol dependent endocytosis: different classes different endocytosis. Goes to the nucleus: transported via the endoplasmic reticulum not microtubule. Takes about 12 hours from attachment to nucleus.
