MBIO 2420 Lecture Notes - Lecture 20: E2F, Retinoblastoma Protein, S Phase
7 Dec 2016
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Cell cycle: r factor - see if cell will go into s phase to replicate dna. E1a affects rb and e2f to make it go into s phase: there is a e2f that blocks go into s phase. E2f 4 and 5 - on the promoter: there is a e2f that makes it into s phase. E2f 1 2 and 3 - makes it go into transcription. On the promoter - turns on gene expression. Makes genes needed to go into s phase. E1a binds to rb to get rid of e2f, and free e2f that causes the cell to go into s phase: rb gets phosphorylated usually to release e2f. E1a action: cr2 binds the b cyclin fold of the prb with very high affinity, brings cr1 close to e2f, motif is, these are e2f 1,2,3 - they activate transcription. Normal state - e2f on promoter with rb: hdacs - transcriptional repressors, repressor e2f.
