MBIO 3410 Lecture Notes - Lecture 6: Chromatin Remodeling, Dna Footprinting, 5.8S Ribosomal Rna
Document Summary
In e. coli, seven different rrn operons called rrna, rrnb, etc. operons are not next to each other. Ribosomal genes and rrna processing in eukaryotes: ribosomal genes tandem repeats of small subunit. 5. 8s and large subunit rrna genes: one promoter controls expression of three genes, prescursor rrna has to be process to yield mature versions of. The rest are silenced involving chromatin remodeling. Locate dna binding sites: may show dna-protein contact points, regulatory regions such as promoters. Dna binding proteins: mapping their contact/binding sites on the dna, dna bound to protein is protected . Prediction of transcript start point and location of promoter. Steps: use radiolabeled dna, lane 3 dna exposed to dna binding protein, lane 3 band does not travel as far. Primer extension: for mapping 5" ends of transcripts, i. e. to get an estimate as to the position of the promoter. Optimized to bind either: 5" end of mrna, 3" end of mrna.