MBIO 3430 Lecture Notes - Lecture 8: Intron, Speciation, Horizontal Gene Transfer

• School: University of Manitoba
• Department: Microbiology
• Course: MBIO 3430
• Professor: Georg Hausner

Document Summary

Ring of life concept: not as accepted as two or three domain theory. Eukaryotes are due to the merger of two genomes (archaea and bacteria: eukaryotes evolved new genes, lost some ancestral genes and required energy producing organelles. 16 rrna a(cid:374)d other ge(cid:374)es are used as (cid:862)(cid:373)arkers(cid:863) for spe(cid:272)ies ide(cid:374)tifi(cid:272)atio(cid:374) Application: rapid species identification without culturing the organism. Also many organisms have never been seen before we only have dna sequences for them. Ribosomal database project (rdp: provides quality controlled, aligned and annotated bacterial and archaeal 16s rrna sequences, and fungal 28s rrna sequences, and a suite of analysis tools for the scientific community. Use 16s or 18s to estimate members of the microbial community: use pcr primers to amplify the rdna sequences, sequence analysis usi(cid:374)g (cid:862)(cid:271)last-like(cid:863) a(cid:374)d phyloge(cid:374)eti(cid:272) a(cid:374)alysis to assign sequences to clades. Hunan skin microbiome: 1-3% of human body mass, outnumber human cells 10 to 1.