MBIO 4440 Lecture Notes - Lecture 8: Sanger Sequencing, Omics, Transcriptomics Technologies

• School: University of Manitoba
• Department: Microbiology
• Course: MBIO 4440
• Professor: Silvia Cardona

Document Summary

In this course, we will see examples of each of the disciplines highlighted in blue. How did it all start: 80s: era of cloning, mapping and sanger sequencing. Map-based shotgun sequencing was the common approach: clone first, map second, sequence at the end . In mapped shotgun sequencing, the genome is fragmented and cloned in large fragments. Each large cloned dna fragment is in turned fragmented, subcloned and sequenced. the dna reads are aligned and overlapping reads are assembled in individual contigs. If the contigs overlap they can be assembled into the dna sequence of the initial large fragment: the whole shotgun genome sequence avoids the cloning of large fragments and mapping. The process starts with random fragmentation of genomic dna, cloning and sequencing: because of the high number of short fragments sequenced that produce dna reads with unknown location the assembly process is more difficult.