MBIO 4600 Lecture Notes - Lecture 13: Integron, Antimicrobial Resistance, Integrase
25 Dec 2015
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Document Summary
Causes random mutations when forming lysogens: no unique bacterial attachment site but inserts randomly in chromosome, cut-and-paste transposition. Replicates by replicative transposition without resolution of the: repetitive replicative transpositions multiple copies of mu concatermers. Packaged into phage heads by making cuts in the flanking host dna. 500-2000 bp from the ends of mu dna: each mu dna molecule has unique host dna end. Useful for transposon mutagenesis; random gene fusions. Systems for acquiring and expressing useful genes. Important for evolution of acquirement of antibiotic resistance genes. Basic integron structure: integrase orf, attachment site integron (atti, promoter located upstream of attachment site. Gene in attachment site transcribed by the promoter. Gene recruitment: antibiotic resistance cassette with attc (attachment site cassette) site and antibiotic resistance gene, integrase recognizes attc, cassette excised and ciricularized, integrase promotes site-specific recombination between attc and atti (cid:1) (cid:1)
