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Lecture 29

MBIO 4600 Lecture 29: Lecture 29

5 Pages

Course Code
MBIO 4600
Brassinga Ann

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Lecture 29 Neisseria gonorrhoeae Genome of strain FA1090 contains many of recombination and repair proteins described for E. coli o RecA, RecBCD o Partial complement of RecF recombination pathway proteins Lack exonuclease and RecF o Partial complement of repair systems described for E. coli Mismatch repair (mutS and mutL but no mutH and dam) Unknown if methyldirected o Multiple copies (3) of genome per cell polyploidy Natural competence of N. gonorrhoeae Competent for DNA uptake in all growth phases Highly efficient Incoming DNA appears to be processed into ssDNA form o Integration via homologous recombination o Requires RecA o Independent of RecF pathway o RecBCD required mutants transformable but at reduced rates Pilation linked to competence
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