MBIO 4612 Lecture Notes - Lecture 8: Sp1 Transcription Factor, Stem-Loop, Intron
9 Feb 2020
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Document Summary
Binds constantly expressed factors that are common to all cells: sp1, nf-1, ccaat. Binds regulatory factors via: response elements, cell-type specific elements, distal elements. Exons + introns: exons are kept after mrna splicing, introns are removed. Terminator sequence: poly a tail dictates stability, rate of translation, etc, highly stable stem loop causes pol to stall + fall off. Goal = unwind ds dna & polymerize mrna. Pol ii = dna-dependent rna pol (can only use dna as template) Assembles into large initiation & elongation complexes capable of: Needs this energy to unwind dna helix downstream. Initiation: pic assembly, open complex, promoter clearance. Elongation: transitions to transcription elongation complex (tec, closed complex. Termination: opens, dissociates from dna complex, open complex. 2 large + 10 small polypeptides = 12 total in human. 2 non-essential + 10 essential = 12 total in yeast.
