MBIO 4612 Lecture Notes - Lecture 9: Tata Box, Transcription Factor Ii B, Transcription Factor Ii D

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Many interactions with pol ii makes pol ii capable of selective initiation in vitro: pol ii + gtfs = 30 polypeptides. Nucleation: tfiid + tata form initial committed complex, tafs+ inr/dpe may also initiate pic assembly, in general core promoter sequence is recognized by a sequence specific gtf. Link: initial committed complex recognized by tfiia+b, when tfiib is bound, the complex becomes accessible to rnapii. Rna pol ii recruitment: assembly of pol ii is assisted by tfiif forms the minimal initiation complex, dabf-rnapolii is not transcriptionally competent. Maturation: recruitment of tfiie+h, this is the complete transcriptionally competent. Clearance & elongation: release of most tfii factors, elongating pol ii leaves the promoter. A universal tf used by all 3 euk rna pols (sl1, tfiid, tfiiib) Binds tata box a key sequence recognition event during pic assembly: binds a variety of different tata-like sequences, slow binding reaction rate limiting step for tata-promoters, binds as monomer in the minor groove.

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