MBIO 4612 Lecture Notes - Lecture 19: Histone Deacetylase, Gene Silencing, Heterochromatin
9 Feb 2020
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Identifies + binds methylated cpg (involved in silencing) different than methylated histones! (those are aa residues, this is dna) Mecp2 + mbd1-4 family of vertebrate proteins: share methyl-cpg-binding domain (mbd, mbd allows binding to single methylated cpg pairs as a monomer, contain a transcriptional repression domain (trd, recruits co-repressors with histone deacetylase (hdac) activity. Part of a multiprotein repression complex that contains hdacs: acts on chromatin near the mcpg to erase acetylation & help silence the methylated gene. One of the 1st unsolved problems in classical genetics seen in eye colour mutants in. Pev = cells with identical genotypes produce different phenotypes in the same organism. Reflects competition between maintenance of an active transcriptional state or the inactive state by heterochromatinization. Pev results from heterochromatin spreading along a chromosome: daughter strands for cell division are produced at different stages of heterochromatin spreading will have different amounts of genes inactivated.
