Class Notes (835,006)
Canada (508,865)
Microbiology (500)
MBIO 3000 (38)
Lecture

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Department
Microbiology
Course
MBIO 3000
Professor
Steven Theriault
Semester
Winter

Description
MBIO3000Guest04012014Missedfirst20minutesstartat850PlasmidscircularpiecesofDNASmalloriginofreplicatiomselectionmarkerMultipleCloningSiteLowmediumandhighcopyplasmidsWhylowlowerfrequencyofmutationDifferenttypeofplasmids250CloningdontneedtobuyagainGeneexpressionRBSetcTransformationtechniqueforputtingtheforeignplasmidintoabacterialhostElectroporationuseelectriccurrentChemicalusegradientandheatMOSTCOMMONMicroinjectionphysicallydeliverDNAplasmidwithaneedleTransfectionsameastransformationinbacteriaexceptforEUKARYOTICcellsGeneguncoatgoldparticleswithDNAandshootitintocellElectroporationChemicalCarboncilicateminineedlescentrifugeandtheypenetrateintothecell
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