MBIO 4020 Lecture Notes - Lecture 15: Listeria Monocytogenes, Canarypox, Recombinant Virus

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Include promoters, translational start signal ect that will be used by host cell. Include signal sequences if ag is to be secreted from cell humoral response. Create a dna molecule encoding an hiv peptide or protein. Expressed in host cells endogenous ag and mhci -> ctl response. Introduce into cells directly, in a bacterial carrier or viral carrier. Attenuated salmonella typhi, mycobacterium tuberculosis (bcg, vaccine organism), Hiv ag to be expressed inside cell so they can be presented with mhci. Recombinant virus containing hiv genes is vaccine. Hiv genes contained in recombinant viruses: vaccinia (smallpox) virus (attenuated, canarypox (no human disease, adenovirus should give mucosal ir, attenuated rabies virus mucosal. Adjuvants non-spcifically increase the ir by controlling ag release or modifying ir. Va(cid:272)(cid:272)i(cid:374)es ge(cid:374)erated a(cid:271) agai(cid:374)st peptide, (cid:271)ut they did(cid:374)"t (cid:271)i(cid:374)d to or (cid:374)eutralize (cid:448)irus. Prevented in vitro infection with test virus, but not primary isolates. Mot (cid:374)eutralizi(cid:374)g a(cid:271) produ(cid:272)ed are(cid:374)"t effe(cid:272)ti(cid:448)e agai(cid:374)st a (cid:449)ide ra(cid:374)ge of (cid:448)irus.

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