MBIO 4600 Lecture Notes - Lecture 19: Dna Repair, Reca, Dna Replication
25 Dec 2015
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Document Summary
Although irradiated phage survived in irradiated e. coli cells, the majority were permanent lysogens (weigle: concluded that induced repair systems were error-prone. Which is the responsible induced repair system: not recombination replication repair or nucleotide excision repair, but reca mutations prevented sos-indcued mutagenesis. Umuc and umud (ultraviolet-induced mutations c and: induced by reca. Autocleavage of lexa and umud (to umud") Icosahedral heads (or capsid) phage genome (rna or dna) Elaborate tail structures penetrate bacterial membranes and cell walls to inject dna. Regulation: detected by plaque formation on susceptible host bacteria, transcriptional = gene expression, post-transcriptional = regulate translation of transcripts (cid:1) (cid:1) (cid:1) Simple program of gene expression: 50 genes early and late classes. After infection, expression of genes proceeds from left to right. Genes include (from left to right): antirestriction, protein kinase, Rna polymerase, dna ligase, dna metabolism genes, and phage assembly genes (including capsid gene) >200 genes; complex regulation of gene expression.
