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Class Notes for Microbiology at University of Manitoba

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MBIO 4440 Lecture Notes - Lecture 10: Sanger Sequencing, Massive Parallel Sequencing, Capillary Electrophoresis
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MBIO 4440 Lecture 10 Sanger Sequencing - Sanger Sequencing is a robust method and after three decades of improvements it can achieve read-lenghts of up to 1,000 bp and per-base accuracies as high as 99.999. - An important ...

Microbiology
MBIO 4440
Silvia Cardona
MBIO 4440 Lecture Notes - Lecture 11: New England Biolabs, Molecular-Weight Size Marker, Deoxyribonuclease I
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MBIO 4440 Lecture 11 DNA Fragmentation - Once the DNA is isolated, achieving the right size of the target DNA fragments in the final library is a key parameter. - Three approaches are available to fragment nucleic acid cha...

Microbiology
MBIO 4440
Silvia Cardona
MBIO 4440 Lecture 9: Microbial Genomics
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MBIO 4440 Lecture 9 A genomic library is a collection of strains that represent the genome of a given organism. The strains harbor different DNA fragments, cloned in a suitable vector. The organisms genomic DNA is extracte...

Microbiology
MBIO 4440
Silvia Cardona
MBIO 4440 Lecture Notes - Lecture 8: Sanger Sequencing, Omics, Transcriptomics Technologies
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MBIO 4440 Lecture 8 What is Genomics? A discipline concerned with sequencing and analyzing genomes And the application of genomic information to diverse fields (omics) The terms associated with omics emphasize the use of w...

Microbiology
MBIO 4440
Silvia Cardona
MBIO 4440 Lecture 6: Vaginal Microbiome
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MBIO 4440 Lecture 6 How the immune system surveils the vaginal microbiota Pattern recognition receptors (PRRs) such as toll-like receptors (TLRs) and NOD factors Microbial stimulation of PRRs initiates cytokinechemokine si...

Microbiology
MBIO 4440
Silvia Cardona
MBIO 4440 Lecture 5: Vaginal Microbiome
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MBIO 4440 Lecture 5 A complex microbiological system: the vaginal microbiome - The assemblages of microbes (microbiota) associated with the human body have been shown to affect human physiology, immunity and nutrition. In ...

Microbiology
MBIO 4440
Silvia Cardona
MBIO 4410 Lecture Notes - Lecture 3: Virus Quantification, Absorbance, Electron Microscope
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MBIO 4410 Lecture 3 Virus Measurement - Biophysical - Biochemical - Molecular Biologic - Biologic quantalquantitative Virus Quantification - The only way is to count viral particles using an electron microscope - On a grid...

Microbiology
MBIO 4410
Kevin M.Coombs
MBIO 4410 Lecture 1:
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find more resources at oneclass.com MBIO 4410 Lecture 1 Course Evaluation - Midterm 30% - Term Paper 30% - Final (Semi cumulative) 40% Current worst viruses Ebola HIV WNV Historical...

Microbiology
MBIO 4410
Kevin M.Coombs
MBIO 4440 Lecture 4: Cell Cycle in Caulobacter
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MBIO 4440 Lecture 4 FROS This system allows tracking any site in the chromosome by time-lapse fluorescence microscopy. The system is based on the delivery of the lac operator site lacO together with a selection marker (in ...

Microbiology
MBIO 4440
Silvia Cardona
MBIO 4440 Lecture Notes - Lecture 2: Dna Replication, Doubling Time, Cell Growth
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MBIO 4440 Lecture 2 - Although each cycle follows the I+C+D rule, if cells replicate at a faster growth rate (in this case the doubling time is 70 min) there should be almost no time for the inter- initiation time. - Howev...

Microbiology
MBIO 4440
Silvia Cardona
MBIO 4440 Lecture Notes - Lecture 3: Caulobacter Crescentus, Lucy Shapiro, Proteobacteria
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MBIO 4440 Lecture 3 Last class, we concluded that studying the bacterial cell cycle using E. coli as the model organism poses two challenges. First, it is difficult to synchronize an E. coli cell culture. Second, within th...

Microbiology
MBIO 4440
Silvia Cardona
MBIO 4440 Lecture Notes - Lecture 1: Microbiology, Cell Division, Cell Growth
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MBIO 4440 Lecture 1 Systems Microbiology (SM) System Microbiology is a field of Microbiology that looks at the interactions between the parts of a microbiological system. The goal is to understand how the system functions ...

Microbiology
MBIO 4440
Silvia Cardona
MBIO 1220 Lecture Notes - Lecture 19: Lyme Disease, Zoonosis, Botulism

Chapter 19 - Epidemiology Basic Concept of Epidemiology Sporadic - Disease occurs only occasionally Ex. Botulism Endemic - disease occurs at fairly constant levels Ex. Malaria, the common cold Epidermic - disease occurs in...

Microbiology
MBIO 1220
Carrie Selin
MBIO 3430 Lecture Notes - Lecture 25: Gene Duplication, Cas1, Natural Selection 2
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MBIO 3430 Lecture 25 CRISPR and Casposons and group II introns (RT) - Casposons are dna transposons that encode protein for the crispr system o Also includes reverse transcriptase - same as the type found in group II intro...

Microbiology
MBIO 3430
Georg Hausner
MBIO 3430 Lecture Notes - Lecture 24: Allopatric Speciation, House Mouse, Polyploid
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MBIO 3430 Lecture 24 Chromosome changes: that prevent pairing due to meiosis (post-zygotic barrier) 1. Chromosome numbers: - Ex. Roberstonian fusions - In many mammals closely related species differ in chromosome numbers -...

Microbiology
MBIO 3430
Georg Hausner

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