ACB 330 Lecture Notes - Lecture 2: Pax6, Exon, Crystallin

• School: U of S
• Department: Anatomy and Cell Biology
• Course: ACB 330
• Professor: Kulyk William

Document Summary

Promoter region: rna polymerase ii binds to nuclease here. Pre-mrna: contains all exons, leader sequences and introns. 7"(cid:373)ethl(cid:455) (cid:272)ap: (cid:272)riti(cid:272)al or mrna to (cid:271)i(cid:374)d to ri(cid:271)oso(cid:373)es. To add this tail, cleave off end, use. Mrna: has only exons + 7 cap +poly a tail. Exon splicing: snrnps form spliceosome which remove intron. Regulation: transcriptionally: gene can be transcribed to be active/inactive, rna processing. Cause huge difference: bcl-x either inhibits or promotes apoptosis bases on the exons: rna stability: longevity of transcripts can vary. Uses micrornas, to tag: translation: mrna may be prevented from matching up with a ribosome, post-translational modifications: Proteolytic cleavage: yields different proteins from single polypetide. Following effects transcriptional activity: interacting of tissue specific transcription factors (tf) with regulatory nucleotide sequences. Tata (tbp) caat (cbf) gc (sp1), tf phosphorylate rna polyii. Ex: delta crystallin & somatostain: both contain pax6. Tf also ca n be location specific. hoxc10: caudal mouse structures.