BMSC 200 Lecture Notes - Lecture 3: Frits Zernike, Dark Field Microscopy, Phase-Contrast Microscopy

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2 Feb 2017
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It always a contrast between the cell and substance surrounding the cell. The power of light microscopy is limited by our ability to magnify. Resolution is the ability to distinguish two adjacent objects as separate and distinct: resolution is determined by the wavelength of light. Inversely proportional to wavelength (shorter wavelength = better resolution: limit of resolution for light microscope is about 0. 2 microunits. At the limit of resolution further magnifaction does not provide any additional detail. Compound light microscope uses visible light to illuminate cells. Many different types of light microscopy: bright field, phase contrast, dark field, fluorescence. Bright field scope: specimens are visualized because of differences in contrast (density) between specimen and surroundings, there are two sets of lenses forming the image. Total magnification = objective x ocular magnification. Invented but frits zernike in 1936: phase ring amplifies differences in the refractive index of cell and surroundings.

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