BIOL101 Lecture Notes - Lecture 7: Hepes, Litmus, Mops

• School: University of Waterloo
• Department: Biology
• Course: BIOL101
• Professor: Bernard Glick

Document Summary

Preparation of buffer solutions: more common approach (real world): start with a pure weak acid and neutralize with ______________ (or neutralize a pure weak base with _____________) In other words, you are performing a ______________ experiment: example: adding 0. 0500 mol solid naoh to a solution of 0. 200 m. Preparation of buffer solutions (additional space for calculation) Buffer capacity: buffers can"t resist changes in ph indefinitely, most effective when ________________, adding strong acids/bases alters this ratio and the ph changes, buffer is destroyed when either ha or a- becomes completely _____________________ Buffer capacity: buffers are better able to resist ph changes when concentrations of ha and a- are both relatively ________ and almost equal to each other, example: comparing a buffer of 0. 100 m ch3cooh / 0. 100 m. Ch3coo- with a buffer of 0. 001 m ch3cooh / 0. 001 m ch3coo- In general, a buffer is considered effective over a ph range corresponding to pka ______