BIOL130 Lecture Notes - Lecture 4: Tata-Binding Protein, Rna Splicing, Tata Box

• School: University of Waterloo
• Department: Biology
• Course: BIOL130
• Professor: Heidi Engelhardt

Document Summary

Unit 5 gene expression dna to rna: central dogma of (molecular) biology dna rna . Protein: genotype effect/ activity of protein (proteome) (change the protein content) phenotype. Recap rna vs. dna: monomers themselves deoxy and ribo, 4 nucleotides thymine in dna and uracil in rna, nucleotides in both include sugar, phosphate and base. 3d structure of dna vs. rna: dna antiparallel, rna- in the same strand 3d structure. Diff types of rna found in the cell. Formation of phosphodiester bonds: enzyme rna polymerase mediates the bond catalyzes the reaction. Rna polymerase holoenzyme for transcription at start site. Dna molecules can adopt higher order structure: allows for compact packaging. Transcription in eukaryotes vs. prokaryotes: tbp (tata binding protein) recognizes promoter sequence, tbp + tfiid distorts helix, allows other factors (tfiia, b, C, etc) to pile on to form (cid:858)t(cid:396)a(cid:374)s(cid:272)(cid:396)iptio(cid:374) i(cid:374)itiatio(cid:374) (cid:272)o(cid:373)ple(cid:454)(cid:859) Tata binding protein subunit of tfiid that recognizes and binds to.