BIOL130 Lecture Notes - Lecture 4: Central Dogma Of Molecular Biology, T Formation, Sigma Factor
23 Nov 2017
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Recall central dogma and recap from unit 2: Modifications to central dogma: ma(cid:374)(cid:455) ge(cid:374)es (cid:272)ode fo(cid:396) na (cid:373)ole(cid:272)ules that a(cid:396)e(cid:374)(cid:859)t (cid:373) na (cid:894)(cid:272)e(cid:374)t(cid:396)al dog(cid:373)a o(cid:374)l(cid:455) fo(cid:272)uses o(cid:374) mrna, see chart all made by transcription. Initiate and terminate at right spot: na pol (cid:373)oves alo(cid:374)g st(cid:396)a(cid:374)d 3(cid:859) 5(cid:859, na s(cid:455)(cid:374)thesized 5(cid:859) 3(cid:859) di(cid:396)e(cid:272)tio(cid:374, what does rna pol do, catalyze formation of phosphodiester bonds that link nucleotides together (form sugar- phosphate backbone of rna chain) To begin transcription, rna pol ii need set of general transcription factors (tfiib, tfiid, etc. ) Initiation: tbp (tata binding protein, subunit of tfiid) recognizes promoter sequence: binding of tbp and tfiid distorts dna helix (bending straw), allows other factors to pile onto. Must unwrap compact dna to simplest form so rna pol can access. Rnapol i, ii, iii interact with different sets of promoters. Rna pols require large team of general transcription factors + Exons (coding sequences) are interrupted by introns (non- coding sequences)
