BIOL239 Lecture Notes - Lecture 7: Start Codon, Sigma Factor, Guanidine

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Promoter and terminator sequential regions in dna (start and stop codons are nested within). mrna transcribes the region between the promoter and the terminator, including the start and stop. Region between start and stop codons is the orf (open reading frame). Reformation of the helix displaces the mrna (forces it out, no need for ssb). Transcription can occur simultaneously along the length of the dna in various regions. Orientation of 5" to 3" is very important because the gene lies between the promoter and terminator in that order. Rho protein destabilizes rna polymerase so that it falls off dna: rho-independent. Specific sequences that base pair with each other are transcribed and then a sequence of adenine (aaaaaa) which causes rapid folding of mrna into a hairpin shape. Weak h-bonds between a:u cause release (breaking) off of the transcript when rna polymerase pauses after hairpin formation.

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