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Topic 15.pdf

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BIOL 240
Josh Neufeld

Fundamentals of Microbiology Biology 140 Course notes Dr Josh D Neufeld Topic 15Learning ObjectivesTo consider methods for controlling microorganisms and appreciate the challenges associated with these methodologiesUntil now we have been considering growth characteristics of microorganisms Now well look at how to inhibit their growth or kill them The methods that are used to inhibit growth or sterilize take advantage of cell sensitivity to heat radiation or chemicals or involve removal of the cells by filtration The components of microbial cells are denatured if the temperature rises high enough resulting in loss of cell viability Figure 261 A given organism will have a characteristic sensitivity to temperature with death occurring more rapidly at higher temperatures There is an exponential relationship between time of heating and extent of killing Decimal reduction time D is the time required for a tenfold reduction in the population density and is not dependent on the initial cell concentrationEndospores are much more resistant to heat than are vegetative cells For this reason heat sterilization is aimed at ensuring that endospores are killed At the autoclave temperature of 121C endospores have a decimal red
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