BIOL266 Lecture Notes - Lecture 3: Sanger Sequencing, Protein Sequencing, Sequence Database
25 Sep 2017
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Central dogma is a significant model to store information (dna, transcription, translation) It has to be turned into cdna first before being sequenced. It is the natural way information flows in the cell. The t are replaced with u (thymine to uracil) 1955: first complete protein sequence (insulin, ryle et al. Amino acid sequence chain was discovered before the sequence of dna was. Protein sequencing didn"t get as big or as fast as dna sequencing did, the focus now is to sequence genomes. 1953: structure of dna (watson & crick) 1960"s and 1970"s: only small rnas were sequenced. Development of cloning and then later pcr & sanger sequencing greatly increased sequencing of dna. Very first database (publically funded): stores all genetic information and can be accessed by many. Simpler techniques in sequencing and automation greatly increased ability to collect nucleotide data. With advancement of molecular and bioinformatic techniques came the desire to sequence entire. X174 (5386 bp) sanger et al.
