BIOL308 Lecture Notes - Lecture 10: Methyltransferase, Tata-Binding Protein, Transfer Rna
16 Jul 2014
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Document Summary
*specific proteins recognize specific sites on na and bind. Dna (rna) - protein interactions - very important! Enzymatic: nuclei acids - substrate, protein - enzymes. Helicases [unwinding parental dna duplex during dna replication], histones, tbp etc. In both transcription [dna binding] and tranlation [rna binding] Binding proteins tend to be positively chargeed [ie. histone] Dna and rna are surrounded by positively charged ions. When protein binds to nucleic acid, positively charged ions are displaced [energy requirement etc] Major is more involved in binding because it is more specific. Describe the cis elements and trans factors involved in the process of replication initiation. Describe how ori sequences are located and studied. Efficient process - it always starts at the defined sequence of base pairs. Replication origin: a stretch of dna necessary and sufficient for beginning of replication. Bacterial and plasmid genomes often have only one [more complicated in eukaryotic cell - multiple origins]
