BIOL308 Lecture Notes - Lecture 14: Holliday Junction, Homologous Recombination, Prokaryote
16 Jul 2014
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Recombination: exchange of genetic material between two dna molecules; causes dna rearrangement. Roles/uses: depends on who/what is causing it" biological and experimental. Creation of new gene/allele combinations (through crossing over during meiosis) Gene mapping [ distance between genes estimated through recombination frequency] Occurs between sequences with a limited stretch of similarity. Mobile dna element moves from one site to another [donor and target site] usually little sequence similarity involved. Genetic exchange between a pair of homologous dna sequences. Between two copies of the same chromosome/sister chromatides. Mostly in dna repair in both prokaryotes and eukaryotes. Introduction of the break in one strand of each homolours dna. Strand invasion: ss reagion from one of the parental strands pairs with the complementary strand from the homologous dna molecule. Outcome: two molecules become connected through crossing dna strands. Follow the allele markers and the strands which are cut:
