Class Notes (836,861)
Canada (509,934)
Biology (2,268)
BIOL 308 (146)
Lecture 16

Lecture 16 con't - BIOL308.pdf

1 Page
Unlock Document

BIOL 308
Bruce Wolff

Lecture 16 contMeasuring gene expression by measuring translationSodium dodecyl sulfate polyacrylamide gel electrophoresis SDS PAGE and western blottingSDS negatively charged detergent binds to hydrophobic protein regions helps protein unfoldingProteins bind lots of SDS in constant ratio to their mass SDS gives them a negative charge proportional to their massequal charge density per unit length protein intrinsic charge is maskedElectrophoresis under denaturing conditions proteins migrate towards the positive electrode when voltage is applied to a gelSeparate on the basis of molecular weight rather than intrinsic chargeProteins detected by different stains gel stained with coomassie brilliant blue silver etcWestern BlottingFirst step is SDS PAGEproteins are denatured by heat and detergent SDS and electrophoresed separating on the basis of sizeThe proteinsare transferred to a membrane western blottingSimilar to DNA in southern and northernThe membrane is incubated with an antib
More Less

Related notes for BIOL 308

Log In


Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.