BIOL308 Lecture Notes - Lecture 16: Primary And Secondary Antibodies

30 views1 pages

Document Summary

Sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page and western blotting. Sds; negatively charged detergent; binds to hydrophobic protein regions - helps protein unfolding. Proteins bind lots of sds in constant ratio to their mass - sds gives them a negative charge proportional to their mass (equal charge density per unit length) - protein intrinsic charge is masked. Electrophoresis under denaturing conditions; proteins migrate towards the positive electrode when voltage is applied to a gel. Separate on the basis of molecular weight rather than intrinsic charge. Proteins detected by different stains: gel stained with coomassie brilliant blue, silver, etc. First step is sds page - proteins are denatured by heat and detergent (sds) and electrophoresed, separating on the basis of size. The proteins are transferred to a membrane - western blotting. The membrane is incubated with an antibody specific for one of the proteins.

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers
Class+
$8 USD/m
Billed $96 USD annually
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
30 Verified Answers

Related Documents