BIOL308 Lecture Notes - Lecture 16: Northern Blot, Southern Blot, Complementary Dna
16 Jul 2014
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Document Summary
Gene; entire dna sequence necessary for the synthesis of a rna molecule or functional polypeptide. It includes coding and regulatory regions [up- and downstream] If there is a particular gene, one of interest, in a particular genome. How many copies of the gene are present in the genome. Information carried by a gene is converted into observable product [definition of gene expression] Expression of our gene of interest is usually assessed by: Observing the abundance of rna transcribed from gene of interest. Observing the amount of protein translated from (this) mrna. Is the gene of interest transcribed or not. If yesis it transcribed more then in control (question of suitable control) If yesdid we find more of the accumulated transcript or not (as mrna is getting degraded ) If yesis transcript viable and carried to the ribosomes for translation (as mrna continues to get degraded )
