BIOL308 Lecture Notes - Lecture 23: Glycolysis, Stra, Lincomycin
4 Feb 2015
School
Department
Course
Professor
Document Summary
The peptidyl transferase reaction: energy required for the cycle of peptide bond formation(s), 1 atp - used by the aminoacyl- trna synthetase to make a high- energy acyl bond which binds amino acid to the trna. The breakage of this high-energy bond will drive the peptidyl tranferase reaction and create the peptide bond: 1 gtp - consumed in the delivery of a charged trna to the. A site of the ribosome by ef-tu: 1 gtp is consumed through the. Termination of translation: requires highly specific molecular signals: termination factors (only one factor in eukaryotic cells, erf1; two factors in prokaryotic cells, Rf1 and rf2: act by recognizing (different) stop codons - release of completed protein chain (folding of the protein is then facilitated by other proteins called chaperones) Rf3: gdp/gtp binding protein which promotes release of rf1/rf2: additional factors promote dissociation of ribosomal components (ribosome recycling factor, rrf, and ef- Nature reviews molecular cell biology 9, 242-253 (march 2008)
