BIOL354 Lecture Notes - Lecture 2: Petri Dish, Cladocera, Chlorophyll
Document Summary
Rapid, cheap, small amounts of solution less waste can be geared (designed) up to a chromic or life cycle test with bio-indicator assessment. 1 to 4 week growth and survival or reproduction. 3 colonies from log phase culture to synthetic growth. 7/14 d old animals feed on fish food. Measurement freeze dried or fresh cells from log phase culture to simple saline medium. Measurement: multi-well petri dish reading using a fluorescence plate reader. Rapid, cheap, small of solution less waste. Use with other bioindicator amounts of solution less waste amounts of solution less waste assessment end-points. Good 1st cut diagnostic (if used prior to further chemical detection) Extended to 18h low sensitivity to contaminants sensitivity to light quality; Edta larger volume of solutions/sediments very labour intensive (hard to see insensitive to many chemicals. Not representative to higher organisms organisms in sediments due to small size) (lower only)
