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Lecture 8

BIOL483- Lecture 8 - Sept 26.docx

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University of Waterloo
BIOL 483
Vivian Dayeh

What is the difference between a finite and a continuous cell line? - Finite cell line is only able to undergo a limited amount of subculturing. Can only passage so many times as it will eventually die - Continuous cell line is an immortal cell line that can be passaged as many times until nutrients run out. Not always derived from abnormal cell. Can come from non cancerous origin. If you’ve taken cells from organism, put them into invitro, they may lose some of their functions. 3 reasons page 23. 1. Dedifferentiation a. LOST IRREVERISBLY! Take a liver (hepatocytes, functioning liver cells) cell and able to see that function in culture dish but for some reason u cant now observe that function, its irreversabily replaced. That is dedifferentiation. 2. Deadaptation a. Lost ability to see that function, but it is regained back if correct cell 3. Selection a. Cells don’t have precursor, don’t differentiate, may have other cells outgrowing. b. In a heterogeneous cell mixture, one cell type may outgrow other cell types c. Maybe the reason why culture isnto showing final differentiated functions. Inducing differentiation IN VITRO - Medium o Supplements help coax the cell - ECM interactions o Mimic what would normally happen in ECM. If you can coat it with some kind of ECM, that may help cell regain abilities or differentiate into another type of cell. - Cell to cell interactions o Good way to get cells to go down lineage.  Example hepatocytes, you may be able to coculturin them with another type of cell and mimic what u would see in natural normal tissue  Maybe hepato lost ability, maybe gorwing them with epithelial cells will help regain hepato ability - Polarity and shape o Cell in tissue may have specific shape, take that same cell and put it in invtro env, they may not have normal shape u see in vivo. o Cell may change shape IN VITRO.  If you can get that shape back, it may differentiate or regain its normal ability. 4 Neoplastic transformation - Two different types, spontaneous and directed - One happens on own, other happens cus you did something Spontaneous - You can have continuous cell line that is not neoplastic cell culture. - If you are culturing cells and these cells may exhibit (after normal culturing) this neoplastic confirmation, its considered spontaneous. Are all neoplastic cells continuous? YES! But not the reverse! Directed - Can use 4 different experimental treatments to intentionally transform to neoplastic cells. How do u tell if cell is neoplastically transformed? - Two sets of criteria o If you have neoplastically transformed cell and take those invitro cell and put it in invivo env, and u get production of tumor = you have neoplastic tr
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