- If you have isolated cells for first time and place it in in vitro env and haven’t split that culture,
that is primary culture
- Have to let them remain in that env for a certain amount of time.
Different ways to isolate cells based on characteristics. Known as cell selection.
- Physical (blood origin)
o Ficoll = helps separate cells in layers
o Great mechanism to isolate cells in blood lineage, helps to isolate cells from pancreas as
- Cell selection during culturing
o More commonly used
o When you have isolated ur cell and place into culture, certain ce