CHEM120L Lecture Notes - Lecture 2: Spectroscopy, Saini, Industrial & Engineering Chemistry Research
2 Feb 2015
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Document Summary
Concentration of fast green and pigmentation in chloroplasts. Spectroscopy is the process of analyzing the relationship between matter and energy. Using a spectrophotometer one can analyze the absorption of a substance in relation to its wavelength. The absorption spectrum of a substance relates the absorption of the substance to its wavelength. Similarly the concentration curve is used to determine the concentration of a substance in a sample by relating wavelength and absorption through beer"s law. Beer"s law states that the amount of light absorbed by a solute is directly proportional to the concentration of the substance (patil, 2009). In experiment one of this lab, fast green solution was diluted with distilled water and those solutions were used in the spectrophotometer. Fast green is a dye that is also a suspected cancer agent (gupta, 2004). In experiment two of the lab the pigments in chloroplasts were isolated through chromatography and then used to determine the wavelengths of chlorophyll a and b.
