CHEM123L Lecture Notes - Lecture 2: Health Informatics, Molar Attenuation Coefficient, Spectrophotometry
11 Jul 2016
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Document Summary
The experiment demonstrated that the percent ethanol of an unknown wine sample could be determined from the standard curve between percent ethanol and absorbance, given that the absorbance of the sample was known. A series of standards of known alcohol concentrations were used to obtain the unknown alcohol content and was done so by a spectrophotometer. The principle behind this experiment is stemmed from. Beer"s law, which quantifies the depletion of light by a material passing through a substance. 3 the reduction in intensity of a light wave is known as absorption. It is given by the formula a= lc, where a is absorbance; is molar absorptivity with units of. L mol1 cm-1; l is the path length of the sample in cm; and c is the molar concentration of the compound in mol l-1. 3. The unknown alcohol concentration of the undiluted wine sample, mendeleev.
