CHEM237L Lecture Notes - Vitamin C, Catalase, Reactive Oxygen Species

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Table 1: enzymatic rates for varying enzyme concentrations. Table 2: enzymatic rates for varying substrate concentrations without ascorbic acid. Table 3: enzymatic rates for varying substrate concentrations with ascorbic acid. Concentrations i e t u n m s e s i r e t a. The figure above represents the data obtained from part a of the experiment, plotting enzymatic rate vs. concentration of catalase. Plots involving only part b data are on the next consecutive pages. Catalase is part of the oxidasereductases reaction type. Catalase breaks down h2o2 which is a harmful byproduct in normal metabolic processes that cells undergo. It protects the cell from oxidative damage by reactive oxygen species because it does not release any free radicles. The graph illustrates that the higher the concentration of enzyme, the faster the enzymatic rate it. By observing the graph, a linear relationship is seen.