CHEM237L Lecture Notes - Chromatography, Cuvette, Myoglobin
15 Jul 2014
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Results: the chromatogram illustrates the two proteins separate in the sample: Chromatogram of the absorbance of 280nm and 410nm light. 0. 05 e c n a b r o s b. 11 and 12 were pale red myoglobin present. 8 and 10 were clear and (+) spot test . Fraction 9 was pale yellow and (+) spot test catalase present. A410: standard curve of bsa to determine concentration of catalase within the sample. Bradford assay standard curve of bsa t h g i l m n. 5 f o e c n a b r o s b. Sample calculation for [bsa] = (0. 120mg/ml * 0. 1ml) 1. 5ml = 0. 008 mg/ml in the cuvette. Calculating the [catalase] in the fraction assayed by using the line of best fit equation from the previous graph: Test tube 6: y = 53. 035x + 0. 0141. Test tube 7: y = 53. 035x + 0. 0141.
