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CHEM 237L (6)
Lecture

Protein Separation and Purification - CHEM237 - Winter2014.pdf

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Department
Chemistry
Course
CHEM 237L
Professor
Laura Marrone
Semester
Winter

Description
PROTEIN SEPARATION AND PURIFICATIONResults 2 The chromatogram illustrates the two proteins separate in the sample Chromatogram of the Absorbance of 280nm and 410nm Light 03Fraction 12 was red Note all fractions not mentioned were clear myoglobin present 11 and 12 were 025pale red myoglobin present 028 and 10 were clearandspot testFraction 9 was pale Catalase present yellow andspot 015test Catalase Absorbancepresent Void volume at 01fraction 735mL 0050024681012141618Fraction Number A280A410 3 Standard Curve of BSA to determine concentration of catalase within the sampleBradford Assay Standard Curve of BSA 05045 04035y53035x00141 R09931 0302502015Absorbance of 595nm light0100500000100020003000400050006000700080009Concentration of BSA mgmLSample Calculation for BSA0120mgmL01mL15mL0008 mgmL in the cuvette
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