CHEM267L Lecture Notes - Lecture 14: Dna Polymerase, Nonlinear Regression, Enzyme Kinetics
5 Jan 2016
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Questions: hydrolase is an enzyme that helps speed up hydrolysis reactions. A hydrolysis reaction is where water is used to help break bonds (voet et al 2013). Alkaline phosphatase is considered as a hydrolase because it breaks the bond between the phosphate and oxygen on p-nitrophenyl phosphate. Alkaline phosphatase basically dephosphorylates the molecule: the relationship between the alkaline phosphatase and the rates determined, a)michaelis menten is the first plot. from the lab are positive. This shows that as the concentration of substrate increases, the enzymatic rate increases as well. This is exactly what was expected due to the substrate concentration and reaction rate being directly proportional. As the concentration of substrate increases, more active sites will be present, meaning the enzyme will have more opportunities to bind, making the reaction faster. The software used to calculate these curves is solver, from the program excel.
