GERON255 Lecture Notes - Lecture 15: Chromosome, Apoptosis, Telomerase
19 Apr 2016
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Document Summary
In connective tissues in the body, produce collagen, elastin, they hold cells together. Broke tissue into single cells with enzyme called trypsin. Grew single cells in flat bottomed dish under good growing conditions. They will divide until the bottom of the dish is covered. Enzyme was added to remove the cells from the dish. This series of repetition is called a serial passage. Hayflick found that this may only be done around 50 serial passages (cid:1) and then cells wont replicate anymore (cid:1) (cid:1) Age of donor: doubling of cells is inversely related to age of donor, # of doublings: 20-30, 80-90 years: this means that the cells in vitro have some form of memory (cid:1) (cid:1) (cid:1) (cid:1) (cid:1) (cid:1) Species specific: mitotic divisions of fetal fibroblasts closely related to the longevity of that species, organism and lifespan and limit: Mouse 3 years 12 doublings. Human 100 years 40-60 doublings. Galapagos tortoise 200 years 140 doublings.
